Some notable unialgal isolates of microalgae from rice paddies of San Juan, Batangas, Philippines
Abstract
Microalgae from soil collected from rice paddies in San Juan, Batangas were identified. Samples of surface soil were diluted with floodwater and plated on BG11 and BG11–N, and the density of the microalgae were estimated to be 1.9 × 105 and 1.5 × 105 colony forming units per mL (CFU/mL), respectively. Unialgal and axenic cultures of the microalgae were isolated by culturing and streaking on solid BG11 and BG11–N media. Unialgal isolates of heterocystous cyanobacteria namely Nostoc humifusum Carmichael ex Bornet & Flahault, Nostoc linckia Bornet ex Bornet & Flahault, Nostoc piscinale Kützing ex Bornet & Flahault, Anabaena sphaerica Bornet & Flahault, and Scytonema hofmanni C. Agardh ex Bornet & Flahault were identified. Other isolates include cyanobacteria species of Oscillatoria, Calothrix, and another Nostoc species while two Chlorophyta species, i.e., Chlorella and Chlamydomonas, were also isolated.
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